Lymphocyte Activation Lab

We are pleased to announce that our latest paper, titled “AutoCoEv-A High-Throughput In Silico Pipeline for Predicting Inter-Protein Coevolution” has been accepted for publication in the International Journal of Molecular Sciences! The article can be found through here. Accompanying scripts can be found at GitHub.

“AutoCoEv – A High-Throughput In Silico Pipeline for Predicting Inter-Protein Coevolution” offers a a user-friendly, open source, computational pipeline, that enables the search of coevolution within a large number of proteins from a predetemrined dataset. By driving 15 individual programs, culminating in CAPS2 as the software for detecting coevolution, AutoCoEv achieves a seamless automation and parallelization of the workflow. Importantly, a patch to the CAPS2 source code is provided to strengthen its statistical output, allowing for multiple comparison corrections and an enhanced analysis of the results.

Thanks to all involved!

We are thrilled to announce that our article titled “A specific hybridisation internalisation probe (SHIP) enables precise live-cell and super-resolution imaging of internalized cargo”, has been published in Scientific Reports. The article can be found here.

The specific hybridization internalisation probe (SHIP) assay, developed for flow cytometry studies, employs a ssDNA fluorescence internalisation probe and a complementary ssDNA quenching probe to unambiguously detect the internalized receptors/cargo. We take advantage of the SHIP assay to study the trafficking of receptor/ligand complexes using B lymphocytes and B cell receptor-mediated antigen internalization as a model system.

Our article has been included in the “Editor’s choice: super-resolution microscopy” which highlights the versatility and significance of this model system.